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The Faculty of Medicine - Microbiology and Molecular Genetics: Rosenshine Ilan

Researchers

 Last updated June 2021 - School of Pharmacy

List of Publications

(1) Baidya AK, Rosenshine I, Ben-Yehuda S. Donor-delivered cell wall hydrolases facilitate nanotube penetration into recipient bacteria. Nat Commun 2020;11(1).

(2) Ramachandran RP, Spiegel C, Keren Y, Danieli T, Melamed-Book N, Pal RR, et al. Mitochondrial targeting of the enteropathogenic escherichia coli map triggers calcium mobilization, adam10-map kinase signaling, and host cell apoptosis. mBio 2020;11(5):1-18.

(3) Gur-Arie L, Eitan-Wexler M, Weinberger N, Rosenshine I, Livnah O. The bacterial metalloprotease NleD selectively cleaves mitogen-activated protein kinases that have high flexibility in their activation loop. J Biol Chem 2020;295(28):9409-9420.

(4) Biran D, Rosenshine I, Ron EZ. Escherichia coli O-antigen capsule (group 4) is essential for serum resistance. Res Microbiol 2020;171(2):99-101.

(5) Elbaz N, Socol Y, Katsowich N, Rosenshine I. Control of type III secretion system effector/chaperone ratio fosters pathogen adaptation to host-adherent lifestyle. mBio 2019;10(5).

(6) Mintz D, Salamon H, Mintz M, Rosenshine I, Shpigel NY. Intraepithelial neutrophils in mammary, urinary and gall bladder infections. Vet Res 2019;50(1).

(7) Kassa EG, Zlotkin-Rivkin E, Friedman G, Ramachandran RP, Melamed-Book N, Weiss AM, et al. Enteropathogenic Escherichia coli remodels host endosomes to promote endocytic turnover and breakdown of surface polarity. PLoS Pathog 2019;15(6).

(8) Pal RR, Baidya AK, Mamou G, Bhattacharya S, Socol Y, Kobi S, et al. Pathogenic E. coli Extracts Nutrients from Infected Host Cells Utilizing Injectisome Components. Cell 2019;177(3):683-696.e18.

(9) Bhattacharya S, Baidya AK, Pal RR, Mamou G, Gatt YE, Margalit H, et al. A Ubiquitous Platform for Bacterial Nanotube Biogenesis. Cell Rep 2019;27(2):334-342.e10.

(10) Ramachandran RP, Vences-Catalán F, Wiseman D, Zlotkin-Rivkin E, Shteyer E, Melamed-Book N, et al. EspH suppresses erk by spatial segregation from CD81 tetraspanin microdomains. Infect Immun 2018;86(10).

(11) Litvak Y, Sharon S, Hyams M, Zhang L, Kobi S, Katsowich N, et al. Epithelial cells detect functional type III secretion system of enteropathogenic Escherichia coli through a novel NF-κB signaling pathway. PLoS Pathog 2017;13(7).

(12) Katsowich N, Elbaz N, Pal RR, Mills E, Kobi S, Kahan T, et al. Host cell attachment elicits posttranscriptional regulation in infecting enteropathogenic bacteria. Science 2017;355(6326):735-739.

(13) Ronin I, Katsowich N, Rosenshine I, Balaban NQ. A long-term epigenetic memory switch controls bacterial virulence bimodality. eLife 2017;6.

(14) Nissim-Eliraz E, Nir E, Shoval I, Marsiano N, Nissan I, Shemesh H, et al. Type three secretion systemdependent microvascular thrombosis and ischemic enteritis in human gut xenografts infected with enteropathogenic Escherichia coli. Infect Immun 2017;85(11).

(15) Rosenshine I. Bacterial pathogenesis: Cooperative immunomodulation. Nat Microbiol 2016;1(7).

(16) Dupont A, Sommer F, Zhang K, Repnik U, Basic M, Bleich A, et al. Age-Dependent Susceptibility to Enteropathogenic Escherichia coli (EPEC) Infection in Mice. PLoS Pathog 2016;12(5).

(17) Yerushalmi G, Litvak Y, Gur-Arie L, Rosenshine I. Dynamics of expression and maturation of the type III secretion system of enteropathogenic Escherichia coli. J Bacteriol 2014;196(15):2798-2806.

(18) Baum M, Watad M, Smith SN, Alteri CJ, Gordon N, Rosenshine I, et al. PafR, a novel transcription regulator, is important for pathogenesis in uropathogenic Escherichia coli. Infect Immun 2014;82(10):4241-4252.

(19) Lai Y, Rosenshine I, Leong JM, Frankel G. Intimate host attachment: Enteropathogenic and enterohaemorrhagic Escherichia coli. Cell Microbiol 2013;15(11):1796-1808.

(20) Padavannil A, Jobichen C, Mills E, Velazquez-Campoy A, Li M, Leung KY, et al. Structure of GrlR-GrlA complex that prevents GrlA activation of virulence genes. Nat Commun 2013;4.

(21) Mills E, Baruch K, Aviv G, Nitzan M, Rosenshine I. Dynamics of the type III secretion system activity of enteropathogenic Escherichia coli. mBio 2013;4(4).

(22) Law RJ, Gur-Arie L, Rosenshine I, Brett Finlay B. In vitro and in vivo model systems for studying enteropathogenic Escherichia coli infections. Cold Spring Harbor Perspect Med 2013;3(3).

(23) Nadler C, Koby S, Peleg A, Johnson AC, Suddala KC, Sathiyamoorthy K, et al. Cycling of Etk and Etp phosphorylation states is involved in formation of group 4 capsule by escherichia coli. PLoS ONE 2012;7(6).

(24) Frankel G, Berger CN, Crepin VF, Baruch K, Mousnier A, Rosenshine I. EspZ of enteropathogenic and enterohemorrhagic escherichia coli regulates type III secretion system protein translocation. mBio 2012;3(5).

(25) Price CTD, Al-Quadan T, Santic M, Rosenshine I, Abu Kwaik Y. Host proteasomal degradation generates amino acids essential for intracellular bacterial growth. Science 2011;334(6062):1553-1557.

(26) Zahavi EE, Lieberman JA, Donnenberg MS, Nitzan M, Baruch K, Rosenshine I, et al. Bundle-forming pilus retraction enhances enteropathogenic Escherichia coli infectivity. Mol Biol Cell 2011;22(14):2436-2447.

(27) Sathiyamoorthy K, Mills E, Franzmann TM, Rosenshine I, Saper MA. The crystal structure of escherichia coli group 4 capsule protein GfcC reveals a domain organization resembling that of Wza. Biochemistry 2011;50(24):5465-5476.

(28) Baruch K, Gur-Arie L, Nadler C, Koby S, Yerushalmi G, Ben-Neriah Y, et al. Metalloprotease type III effectors that specifically cleave JNK and NF-κB. EMBO J 2011;30(1):221-231.

(29) Golan L, Gonen E, Yagel S, Rosenshine I, Shpigel NY. Enterohemorrhagic Escherichia coli induce attaching and effacing lesions and hemorrhagic colitis in human and bovine intestinal xenograft models. DNM Dis Models Mech 2011;4(1):86-94.

(30) Elazar S, Gonen E, Livneh-Kol A, Rosenshine I, Shpigel NY. Essential role of neutrophils but not mammary alveolar macrophages in a murine model of acute Escherichia coli mastitis. Vet Res 2010;41(4).

(31) Simovitch M, Sason H, Cohen S, Zahavi EE, Melamed-Book N, Weiss A, et al. EspM inhibits pedestal formation by enterohaemorrhagic Escherichia coli and enteropathogenic E. coli and disrupts the architecture of a polarized epithelial monolayer. Cell Microbiol 2010;12(4):489-505.

(32) Elazar S, Gonen E, Livneh-Kol A, Rosenshine I, Shpigel NY. Neutrophil recruitment in endotoxin-induced murine mastitis is strictly dependent on mammary alveolar macrophages. Vet Res 2010;41(1).

(33) Nadler C, Baruch K, Kobi S, Mills E, Haviv G, Farago M, et al. The type III secretion effector NleE inhibits NF-κB activation. PLoS Pathog 2010;6(1).

(34) Enninga J, Rosenshine I. Imaging the assembly, structure and activity of type III secretion systems. Cell Microbiol 2009;11(10):1462-1470.

(35) Rokney A, Shagan M, Kessel M, Smith Y, Rosenshine I, Oppenheim AB. E. coli Transports Aggregated Proteins to the Poles by a Specific and Energy-Dependent Process. J Mol Biol 2009;392(3):589-601.

(36) Golan L, Livneh-Kol A, Gonen E, Yagel S, Rosenshine I, Shpigel NY. Mycobacterium avium paratuberculosis invades human small-intestinal goblet cells and elicits inflammation. J Infect Dis 2009;199(3):350-354.

(37) Sason H, Milgrom M, Weiss AM, Melamed-Book N, Balla T, Grinstein S, et al. Enteropathogenic escherichia coli subverts phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate upon epithelial cell infection. Mol Biol Cell 2009;20(1):544-555.

(38) Yerushalmi G, Nadler C, Berdichevski T, Rosenshine I. Mutational analysis of the locus of enterocyte effacement-encoded regulator (Ler) of enteropathogenic Escherichia coli. J Bacteriol 2008;190(23):7808-7818.

(39) Shifrin Y, Peleg A, Ilan O, Nadler C, Kobi S, Baruch K, et al. Transient shielding of intimin and the type III secretion system of enterohemorrhagic and enteropathogenic Escherichia coli by a group 4 capsule. J Bacteriol 2008;190(14):5063-5074.

(40) Grinberg N, Elazar S, Rosenshine I, Shpigel NY. β-hydroxybutyrate abrogates formation of bovine neutrophil extracellular traps and bactericidal activity against mammary pathogenic Escherichia coli. Infect Immun 2008;76(6):2802-2807.

(41) Padalon-Brauch G, Hershberg R, Elgrably-Weiss M, Baruch K, Rosenshine I, Margalit H, et al. Small RNAs encoded within genetic islands of Salmonella typhimurium show host-induced expression and role in virulence. Nucleic Acids Res 2008;36(6):1913-1927.

(42) Mills E, Baruch K, Charpentier X, Kobi S, Rosenshine I. Real-Time Analysis of Effector Translocation by the Type III Secretion System of Enteropathogenic Escherichia coli. Cell Host and Microbe 2008;3(2):104-113.

(43) Meyer TF, Rosenshine I. Editorial overview. Curr Opin Microbiol 2008;11(1):1-2.

(44) Shpigel NY, Elazar S, Rosenshine I. Mammary pathogenic Escherichia coli. Curr Opin Microbiol 2008;11(1):60-65.

(45) Gonen E, Vallon-Eberhard A, Elazar S, Harmelin A, Brenner O, Rosenshine I, et al. Toll-like receptor 4 is needed to restrict the invasion of Escherichia coli P4 into mammary gland epithelial cells in a murine model of acute mastitis. Cell Microbiol 2007;9(12):2826-2838.

(46) Mundy R, Petrovska L, Smollett K, Simpson N, Wilson RK, Yu J, et al. Erratum: Identification of a novel Citrobacter rodentium type III secreted protein, EspI, and roles of this and other secreted proteins in infection (Infection and Immunity (2004) 72, 4, (2288-2302). Infect Immun 2007;75(7):3686.

(47) Jobichen C, Li M, Yerushalmi G, Yih WT, Mok Y-, Rosenshine I, et al. Structure of GrlR and the implication of its EDED motif in mediating the regulation of type III secretion system in EHEC. PLoS Pathog 2007;3(5):0639-0646.

(48) Li M, Rosenshine I, Yu HB, Nadler C, Mills E, Hew CL, et al. Identification and characterization of NleI, a new non-LEE-encoded effector of enteropathogenic Escherichia coli (EPEC). Microbes Infect 2006;8(14-15):2890-2898.

(49) Nadler C, Shifrin Y, Nov S, Kobi S, Rosenshine I. Characterization of enteropathogenic Escherichia coli mutants that fail to disrupt host cell spreading and attachment to substratum. Infect Immun 2006;74(2):839-849.

(50) Elgrably-Weiss M, Schlosser-Silverman E, Rosenshine I, Altuvia S. DeoT, a DeoR-type transcriptional regulator of multiple target genes. FEMS Microbiol Lett 2006;254(1):141-148.

(51) Peleg A, Shifrin Y, Ilan O, Nadler-Yona C, Nov S, Koby S, et al. Identification of an Escherichia coli operon required for formation of the O-antigen capsule. J Bacteriol 2005;187(15):5259-5266.

(52) Tan YP, Zheng J, Tung SL, Rosenshine I, Leung KY. Role of type III secretion in Edwardsiella tarda virulence. Microbiology 2005;151(7):2301-2313.

(53) Berdichevsky T, Friedberg D, Nadler C, Rokney A, Oppenheim A, Rosenshine I. Ler is a negative autoregulator of the LEE1 operon in enteropathogenic Escherichia coli. J Bacteriol 2005;187(1):349-357.

(54) Qimron U, Madar N, Mittrücker H-, Zilka A, Yosef I, Bloushtain N, et al. Identification of Salmonella typhimurium genes responsible for interference with peptide presentation on MHC class I molecules: Δ yej Salmonella mutants induce superior CD8+ T-cell responses. Cell Microbiol 2004;6(11):1057-1070.

(55) Li M, Rosenshine I, Tung SL, Wang XH, Friedberg D, Hew CL, et al. Comparative proteomic analysis of extracellular proteins of enterohemorrhagic and enteropathogenic Escherichia coli strains and their ihf and ler mutants. Appl Environ Microbiol 2004;70(9):5274-5282.

(56) Mundy R, Petrovska L, Smollett K, Simpson N, Wilson RK, Yu J, et al. Identification of A Novel Citrobacter rodentium Type III Secreted Protein, EspI, and Roles of This and Other Secreted Proteins in Infection. Infect Immun 2004;72(4):2288-2302.

(57) Marchès O, Ledger TN, Boury M, Ohara M, Tu X, Goffaux F, et al. Enteropathogenic and enterohaemorrhagic Escherichia coli deliver a novel effector called Cif, which blocks cell cycle G2/M transition. Mol Microbiol 2003;50(5):1553-1567.

(58) Yona-Nadler C, Umanski T, Aizawa S-, Friedberg D, Rosenshine I. Integration host factor (IHF) mediates repression of flagella in enteropathogenic and enterohaemorrhagic Escherichia coli. Microbiology 2003;149(4):877-884.

(59) Tu X, Nisan I, Yona C, Hanski E, Rosenshine I. EspH, a new cytoskeleton-modulating effector of enterohaemorrhagic and enteropathogenic Escherichia coli. Mol Microbiol 2003;47(3):595-606.

(60) Creasey EA, Friedberg D, Shaw RK, Umanski T, Knutton S, Rosenshine I, et al. CesAB is an enteropathogenic Escherichia coli chaperone for the type-III translocator proteins EspA and EspB. Microbiology 2003;149(12):3639-3647.

(61) Elgrably-Weiss M, Park S, Schlosser-Silverman E, Rosenshine I, Imlay J, Altuvia S. A Salmonella enterica serovar Typhimurium hemA mutant is highly susceptible to oxidative DNA damage. J Bacteriol 2002;184(14):3774-3784.

(62) Shifrin Y, Kirschner J, Geiger B, Rosenshine I. Enteropathogenic Escherichia coli induces modification of the focal adhesions of infected host cells. Cell Microbiol 2002;4(4):235-243.

(63) Valinsky L, Nisan I, Tu X, Nisan G, Rosenshine I, Hanski E, et al. A host-specific virulence protein of Erwinia herbicola pv. gypsophilae is translocated into human epithelial cells by the type III secretion system of enteropathogenic Escherichia coli. Mol Plant Pathol 2002;3(2):97-101.

(64) Umanski T, Rosenshine I, Friedberg D. Thermoregulated expression of virulence genes in enteropathogenic Escherichia coli. Microbiology 2002;148(9):2735-2744.

(65) Daniell SJ, Takahashi N, Wilson R, Friedberg D, Rosenshine I, Booy FP, et al. The filamentous type III secretion translocon of enteropathogenic Escherichia coli. Cell Microbiol 2001;3(12):865-871.

(66) Tu X, Nisan I, Miller JF, Hanski E, Rosenshine I. Construction of mini-Tn5cyaA′ and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica. FEMS Microbiol Lett 2001;205(1):119-123.

(67) Ozeri V, Rosenshine I, Ben-Ze'Ev A, Bokoch GM, Jou T-, Hanski E. De novo formation of focal complex-like structures in host cells by invading streptococci. Mol Microbiol 2001;41(3):561-573.

(68) DeVinney R, Nisan I, Ruschkowski S, Rosenshine I, Finlay BB. Tir tyrosine phosphorylation and pedestal formation are delayed in enteropathogenic Escherichia coli sepZ::TnphoA mutant 30-5-1(3). Infect Immun 2001;69(1):559-563.

(69) Schlosser-Silverman E, Elgrably-Weiss M, Rosenshine I, Kohen R, Altuvia S. Characterization of Escherichia coli DNA lesions generated within J774 macrophages. J Bacteriol 2000;182(18):5225-5230.

(70) Rosenshine I, Knutton S, Frankel G. Interaction of enteropathogenic Escherichia coli with host cells. Subcell Biochem 2000;33:21-45.

(71) Ilan O, Bloch Y, Frankel G, Ullrich H, Geider K, Rosenshine I. Protein tyrosine kinases in bacterial pathogens are associated with virulence and production of exopolysaccharide. EMBO J 1999;18(12):3241-3248.

(72) Frankel G, Phillips AD, Rosenshine I, Dougan G, Kaper JB, Knutton S. Enteropathogenic and enterohaemorrhagic Escherichia coli: More subversive elements. Mol Microbiol 1998;30(5):911-921.

(73) Rosenshine I. 9.3 Cytoskeletal Rearrangements Induced by Bacterial Pathogens. Methods Microbiol 1998;27(C):499-507.

(74) Ozeri V, Rosenshine I, Mosher DF, Fässler R, Hanski E. Roles of integrins and fibronectin in the entry of Streptococcus pyogenes into cells via protein F1. Mol Microbiol 1998;30(3):625-637.

(75) Rosenshine I. Species specificity and tissue tropism of EPEC and related pathogens. Trends Microbiol 1998;6(10):388.

(76) Wolff C, Nisan I, Hanski E, Frankel G, Rosenshine I. Protein translocation into host epithelial cells by infecting enteropathogenic Escherichia coli. Mol Microbiol 1998;28(1):143-155.

(77) Nisan I, Wolff C, Hanski E, Rosenshine I. Interaction of Enteropathogenic Escherichia coli with Host Epithelial Cells. Folia Microbiol 1998;43(3):247-252.

(78) Ben-Ami G, Ozeri V, Hanski E, Hofmann F, Aktories K, Hahn KM, et al. Agents that inhibit Rho, Rac, and Cdc42 do not block formation of actin pedestals in HeLa cells infected with enteropathogenic Escherichia coli. Infect Immun 1998;66(4):1755-1758.

(79) Rosenshine I. Signal transduction between enteropathogenic Escherichia coli and host epithelial cells. ANN INST PASTEUR ACTUAL 1997;8(2):157-161.

(80) Finlay BB, Kenny B, Stein M, Reinscheid D, Ruschkowski S, Rosenshine I. Molecular mechanisms of enteropathogenic E. coli: Signal transduction, pedestal formation, intimate contact, and diarrhea. Rev Microbiol 1996;27(SUPPL. 1):95-98.

(81) Rosenshine I, Ruschkowski S, Brett Finlay B. Expression of attaching/effacing activity by enteropathogenic Escherichia coli depends on growth phase, temperature, and protein synthesis upon contact with epithelial cells. Infect Immun 1996;64(3):966-973.

(82) Tang P, Rosenshine I, Cossart P, Finlay BB. Listeriolysin O activates mitogen-activated protein kinase in eucaryotic cells. Infect Immun 1996;64(6):2359-2361.

(83) Rosenshine I, Ruschkowski S, Stein M, Reinscheid DJ, Mills SD, Finlay BB. A pathogenic bacterium triggers epithelial signals to form a functional bacterial receptor that mediates actin pseudopod formation. EMBO J 1996;15(11):2613-2624.

(84) Finlay BB, Ruschkowski S, Kenny B, Stein M, Reinscheid DJ, Stein MA, et al. Enteropathogenic E. coli exploitation of host epithelial cells. ANN NEW YORK ACAD SCI 1996;797:26-31.

(85) Andreev J, Borovsky Z, Rosenshine I, Rottem S. Invasion of HeLa cells by Mycoplasma penetrans and the induction of tyrosine phosphorylation of a 145-kDa host cell protein. FEMS Microbiol Lett 1995;132(3):189-194.

(86) Foubister V, Rosenshine I, Finlay BB. A diarrheal pathogen, enteropathogenic escherichia coil (EPEC), triggers a flux of inositol phosphates in infected epithelial cells. J Exp Med 1994;179(3):993-998.

(87) Tang P, Rosenshine I, Finlay BB. Listeria monocytogenes, an invasive bacterium, stimulates MAP kinase upon attachment to epithelial cells. Mol Biol Cell 1994;5(4):455-464.

(88) Rosenshine I, Ruschkowski S, Finlay BB. Inhibitors of cytoskeletal function and signal transduction to study bacterial invasion. Methods Enzymol 1994;236(C):467-476.

(89) Foubister V, Rosenshine I, Donnenberg MS, Finlay BB. The eaeB gene of enteropathogenic Escherichia coli is necessary for signal transduction in epithelial cells. Infect Immun 1994;62(7):3038-3040.

(90) Rosenshine I, Ruschkowski S, Foubister V, Finlay BB. Salmonella typhimurium invasion of epithelial cells: Role of induced host cell tyrosine protein phosphorylation. Infect Immun 1994;62(11):4969-4974.

(91) Rosenshine I, Finlay BB. Salmonella's deceiving signals. J Pediatr Gastroenterol Nutr 1993;17(4):453-454.

(92) Altmeyer RM, McNern JK, Bossio JC, Rosenshine I, Finlay BB, Galán JE. Cloning and molecular characterization of a gene involved in Salmonella adherence and invasion of cultured epithelial cells. Mol Microbiol 1993;7(1):89-98.

(93) Canil C, Rosenshine I, Ruschkowski S, Donnenberg MS, Kaper JB, Finlay BB. Enteropathogenic Escherichia coli decreases the transepithelial electrical resistance of polarized epithelial monolayers. Infect Immun 1993;61(7):2755-2762.

(94) Ruschkowski S, Rosenshine I, Brett Finlay B. Salmonella typhimurium induces an inositol phosphate flux in infected epithelial cells. FEMS Microbiol Lett 1992;95(2-3):121-126.

(95) Rosenshine I, Duronio V, Finlay BB. Tyrosine protein kinase inhibitors block invasin-promoted bacterial uptake by epithelial cells. Infect Immun 1992;60(6):2211-2217.

(96) Finlay BB, Rosenshine I, Donnenberg MS, Kaper JB. Cytoskeletal composition of attaching and effacing lesions associated with enteropathogenic Escherichia coli adherence to HeLa cells. Infect Immun 1992;60(6):2541-2543.

(97) Rosenshine I, Donnenberg MS, Kaper JB, Finlay BB. Signal transduction between enteropathogenic Escherichia coli (EPEC) and epithelial cells: EPEC induces tyrosine phosphorylation of host cell proteins to initiate cytoskeletal rearrangement and bacterial uptake. EMBO J 1992;11(10):3551-3560.

(98) Rosenshine I, Mevarech M. Isolation and partial characterization of plasmids found in three Halobacterium volcanii isolates. Can J Microbiol 1989;35(1):92-95.

(99) Rosenshine I, Tchelet R, Mevarech M. The mechanism of DNA transfer in the mating system of an archaebacterium. Science 1989;245(4924):1387-1389.

(100) Zusman T, Rosenshine I, Boehm G, Jaenicke R, Leskiw B, Mevarech M. Dihydrofolate reductase of the extremely halophilic archaebacterium Halobacterium volcanii. The enzyme and its coding gene. J Biol Chem 1989;264(32):18878-18883.

(101) Rosenshine I, Zusman T, Werczberger R, Mevarech M. Amplification of specific DNA sequences correlates with resistance of the archaebacterium Halobacterium volcanii to the dihydrofolate reductase inhibitors trimethoprim and methotrexate. Mol Gen Genet 1987;208(3):518-522.