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The Faculty of Medicine - Medical Neurobiology: Lichtstein David


Last updated September 2023 - Medical Neurobiology

List of Publications


Zilberstein A, Krivoy N, Horesh N, Klein E, Lichtstein D. Beneficial effect of digoxin-specific Fab fragments in bipolar disorder- a preliminary study. Journal of Affective Disorders Reports [Internet]. 2023;13. Available from:


Sampath V, Horesh N, Sasi B, Zannadeh H, Pogodin I, Singh SV, et al. Synthesis and Biological Evaluation of Novel Bufalin Derivatives. International Journal of Molecular Sciences [Internet]. 2022;23(7). Available from:


El-Mallakh RS, Sampath VP, Horesh N, Lichtstein D. Endogenous Cardiac Steroids in Bipolar Disorder: State of the Art. International Journal of Molecular Sciences [Internet]. 2022;23(3). Available from:


Shin EJ, Nguyen BT, Jeong JH, Hoai Nguyen BC, Tran NKC, Sharma N, et al. Ouabain inhibitor rostafuroxin attenuates dextromethorphan-induced manic potential. Food and Chemical Toxicology [Internet]. 2021;158. Available from:


Singh SV, Fedorova OV, Wei W, Rosen H, Horesh N, Ilani A, et al. Na+, k+-atpase α isoforms and endogenous cardiac steroids in prefrontal cortex of bipolar patients and controls. International Journal of Molecular Sciences [Internet]. 2020;21(16):1–16. Available from:


Leibovich H, Buzaglo N, Tsuriel S, Peretz L, Caspi Y, Katz B, et al. Abnormal Reinnervation of Denervated Areas Following Nerve Injury Facilitates Neuropathic Pain. Cells [Internet]. 2020;9(4). Available from:


Hodes A, Rosen H, Cohen-Ben Ami H, Lichtstein D. Na+, K+-ATPase α3 isoform in frontal cortex GABAergic neurons in psychiatric diseases. Journal of Psychiatric Research [Internet]. 2019;115:21–8. Available from:


Gross NB, Abad N, Lichtstein D, Taron S, Aparicio L, Fonteh AN, et al. Endogenous Na+, K+-ATPase inhibitors and CSF [Na+] contribute to migraine formation. PLoS ONE [Internet]. 2019;14(6). Available from:


Buzaglo N, Golomb M, Rosen H, Beeri R, Ami HCB, Langane F, et al. Augmentation of Ouabain-Induced Increase in Heart Muscle Contractility by Akt Inhibitor MK-2206. Journal of Cardiovascular Pharmacology and Therapeutics [Internet]. 2019;24(1):78–89. Available from:


Lichtstein D, Ilani A, Rosen H, Horesh N, Singh SV, Buzaglo N, et al. Na+, K+-ATPase signaling and bipolar disorder. International Journal of Molecular Sciences [Internet]. 2018;19(8). Available from:


Hodes A, Lifschytz T, Rosen H, Cohen Ben-Ami H, Lichtstein D. Reduction in endogenous cardiac steroids protects the brain from oxidative stress in a mouse model of mania induced by amphetamine. Brain Research Bulletin [Internet]. 2018;137:356–62. Available from:


Hodes A, Rosen H, Deutsch J, Lifschytz T, Einat H, Lichtstein D. Endogenous cardiac steroids in animal models of mania. Bipolar Disorders [Internet]. 2016;18(5):451–9. Available from:


Buzaglo N, Rosen H, Ami HCB, Inbal A, Lichtstein D. Essential opposite roles of ERK and akt signaling in cardiac steroid-induced increase in heart contractility. Journal of Pharmacology and Experimental Therapeutics [Internet]. 2016;357(2):345–56. Available from:


Leinonen JV, Korkus-Emanuelov A, Wolf Y, Milgrom-Hoffman M, Lichtstein D, Hoss S, et al. Macrophage precursor cells from the left atrial appendage of the heart spontaneously reprogram into a C-kit +/CD45 - Stem cell-like phenotype. International Journal of Cardiology [Internet]. 2016;209:296–306. Available from:


Dvela-Levitt M, Cohen-Ben Ami H, Rosen H, Ornoy A, Hochner-Celnikier D, Granat M, et al. Reduction in maternal circulating ouabain impairs offspring growth and kidney development. Journal of the American Society of Nephrology [Internet]. 2015;26(5):1103–14. Available from:


Dvela-Levitt M, Ami HCB, Rosen H, Shohami E, Lichtstein D. Ouabain improves functional recovery following traumatic brain injury. Journal of Neurotrauma [Internet]. 2014;31(23):1942–7. Available from:


Hodes A, Lichtstein D. Natriuretic hormones in brain function. Frontiers in Endocrinology [Internet]. 2014;5(NOV). Available from:


Nesher M, Bai Y, Li D, Rosen H, Lichtstein D, Liu L. Interaction of atrial natriuretic peptide and ouabain in the myocardium. Canadian Journal of Physiology and Pharmacology [Internet]. 2012;90(10):1386–93. Available from:


Fridman E, Lichtstein D, Rosen H. Formation of new high density glycogen-microtubule structures is induced by cardiac steroids. Journal of Biological Chemistry [Internet]. 2012;287(9):6518–29. Available from:


Lichtstein D, Rosen H, Dvela M. Cardenolides and bufadienolides as hormones: What is missing? American Journal of Physiology - Renal Physiology [Internet]. 2012;302(8):F957–8. Available from:


Dvela M, Rosen H, Ben-Ami HC, Lichtstein D. Endogenous ou0061bain regulates cell viability. American Journal of Physiology - Cell Physiology [Internet]. 2012;302(2):C442–52. Available from:


Goldstein I, Lax E, Gispan-Herman I, Ovadia H, Rosen H, Yadid G, et al. Neutralization of endogenous digitalis-like compounds alters catecholamines metabolism in the brain and elicits anti-depressive behavior. European Neuropsychopharmacology [Internet]. 2012;22(1):72–9. Available from:


Feldmann T, Shahar M, Baba A, Matsuda T, Lichtstein D, Rosen H. The Na+/Ca2+-exchanger: An essential component in the mechanism governing cardiac steroid-induced slow Ca2+ oscillations. Cell Calcium [Internet]. 2011;50(5):424–32. Available from:


Nesher M, Shpolansky U, Viola N, Dvela M, Buzaglo N, Ben-Ami HC, et al. Ouabain attenuates cardiotoxicity induced by other cardiac steroids. British Journal of Pharmacology [Internet]. 2010;160(2):346–54. Available from:


Guttmann-Rubinstein L, Lichtstein D, Ilani A, Gal-Moscovici A, Scherzer P, Rubinger D. Evidence of a parathyroid hormone-independent chronic effect of estrogen on renal phosphate handling and sodium-dependent phosphate cotransporter type IIa expression. Hormone and Metabolic Research [Internet]. 2010;42(4):230–6. Available from:


Jaiswal MK, Dvela M, Lichtstein D, Mallick BN. Endogenous ouabain-like compounds in locus coeruleus modulate rapid eye movement sleep in rats. Journal of Sleep Research [Internet]. 2010;19(1 PART. 2):183–91. Available from:


Nesher M, Dvela M, Igbokwe VU, Rosen H, Lichtstein D. Physiological roles of endogenous ouabain in normal rats. American Journal of Physiology - Heart and Circulatory Physiology [Internet]. 2009;297(6):H2026–34. Available from:


Klar A, Haver E, Lichtstein D, Hurvitz H, Foah-Shauli T. Atrial natriuretic peptide in young and elderly children with mild gastroenteritis. Gastroenterology Research and Practice [Internet]. 2009; Available from:


Goldstein I, Lerer E, Laiba E, Mallet J, Mujaheed M, Laurent C, et al. Association Between Sodium- and Potassium-Activated Adenosine Triphosphatase α Isoforms and Bipolar Disorders. Biological Psychiatry [Internet]. 2009;65(11):985–91. Available from:


Nesher M, Vachutinsky Y, Fridkin G, Schwarz Y, Sasson K, Fridkin M, et al. Reversible pegylation prolongs the hypotensive effect of atrial natriuretic peptide. Bioconjugate Chemistry [Internet]. 2008;19(1):342–8. Available from:


Dvela M, Rosen H, Feldmann T, Nesher M, Lichtstein D. Diverse biological responses to different cardiotonic steroids. Pathophysiology [Internet]. 2007;14(3–4):159–66. Available from:


Feldmann T, Glukmann V, Medvenev E, Shpolansky U, Galili D, Lichtstein D, et al. Role of endosomal Na+-K+-ATPase and cardiac steroids in the regulation of endocytosis. American Journal of Physiology - Cell Physiology [Internet]. 2007;293(3):C885–96. Available from:


Nesher M, Shpolansky U, Rosen H, Lichtstein D. The digitalis-like steroid hormones: New mechanisms of action and biological significance. Life Sciences [Internet]. 2007;80(23):2093–107. Available from:


Rosen H, Glukmann V, Feldmann T, Fridman E, Lichtstein D. Short-term effects of cardiac steroids on intracellular membrane traffic in neuronal NT2 cells. Cellular and Molecular Biology [Internet]. 2006;52(8):78–86. Available from:


Goldstein I, Levy T, Galili D, Ovadia H, Yirmiya R, Rosen H, et al. Involvement of Na+, K+-ATPase and Endogenous Digitalis-Like Compounds in Depressive Disorders. Biological Psychiatry [Internet]. 2006;60(5):491–9. Available from:


Deutsch J, Jang HG, Mansur N, Ilovich O, Shpolansky U, Galili D, et al. 4-(3′α15′β-dihydroxy-5′β-estran- 17′β-yl)furan-2-methyl alcohol: An anti-digoxin agent with a novel mechanism of action. Journal of Medicinal Chemistry [Internet]. 2006;49(2):600–6. Available from:


Haviv M, Haver E, Lichtstein D, Hurvitz H, Klar A. Atrial natriuretic peptide in children with pneumonia. Pediatric Pulmonology [Internet]. 2005;40(4):306–9. Available from:


Landsman A, Lichtstein D, Ilani A. Distinctive features of dietary phosphate supply. Journal of Applied Physiology [Internet]. 2005;99(3):1214–9. Available from:


Rosen H, Glukhman V, Feldmann T, Fridman E, Lichtstein D. Cardiac Steroids Induce Changes in Recycling of the Plasma Membrane in Human NT2 Cells. Molecular Biology of the Cell [Internet]. 2004;15(3):1044–54. Available from:


Lichtstein D, Rosen H. Endogenous digitalis-like Na+, K+-ATPase inhibitors, and brain function. Neurochemical Research [Internet]. 2001;26(8–9):971–8. Available from:


Landsman A, Lichtstein D, Bacaner M, Ilani A. Dietary phosphate-dependent growth is not mediated by changes in plasma phosphate concentration. British Journal of Nutrition [Internet]. 2001;86(2):217–23. Available from:


Cheng Q, Lichtstein D, Russell P, Zigler Jr. JS. Use of lipophilic cation to monitor electrical membrane potential in the intact rat lens. Investigative Ophthalmology and Visual Science [Internet]. 2000;41(2):482–7. Available from:


Traub N, Lichtstein D. The Mood Cycle Hypothesis: Possible Involvement Of Steroid Hormones In Mood Regulation By Means Of Na+,K+-Atpase Inhibition. Journal of Basic and Clinical Physiology and Pharmacology [Internet]. 2000;11(4):375–94. Available from:


Lichtstein D, Mcgowan MH, Russell P, Carper DA. Digitalis and Digitalislike Compounds Down-Regulate Gene Expression of the Intracellular Signaling Protein 14-3-3 in Rat Lens. Hypertension Research - Clinical and Experimental [Internet]. 2000;23. Available from:


McGowan MH, Russell P, Carper DA, Lichtstein D. Na+, K+-ATPase inhibitors down-regulate gene expression of the intracellular signaling protein 14-3-3 in rat lens. Journal of Pharmacology and Experimental Therapeutics [Internet]. 1999;289(3):1559–63. Available from:


Lichtstein D, Levy T, Deutsch J, Steinitz M, Zigler Jr. JS, Russell P. The effects of digitalis-like compounds on rat lenses. Investigative Ophthalmology and Visual Science [Internet]. 1999;40(2):407–13. Available from:


Lichtstein D, Steinitz M, Gati I, Samuelov S, Deutsch J, Orly J. Biosynthesis of digitalis-like compounds in rat adrenal cells: Hydroxycholesterol as possible precursor. Life Sciences [Internet]. 1998;62(23):2109–26. Available from:


Lichtstein D, Steinitz M, Gati I, Samuelov S, Deutsch J, Orly J. Bufodienolides as Endogenous Na+, K+-ATPase Inhibitors: Biosynthesis in Bovine and Rat Adrenals. Clinical and Experimental Hypertension [Internet]. 1998;20(42130):573–9. Available from:


Morris JF, Ismail-Beigi F, Butler Jr. VP, Gati I, Lichtstein D. Ouabain-sensitive Na+,K+-ATPase activity in toad brain. Comparative Biochemistry and Physiology - A Physiology [Internet]. 1997;118(3):599–606. Available from:


Furman S, Lichtstein D, Ilani A. Sodium-dependent transport of phosphate in neuronal and related cells. Biochimica et Biophysica Acta - Biomembranes [Internet]. 1997;1325(1):34–40. Available from:


Samuelov S, Lichtstein D. Digitalis-like compounds and Na+, K+-ATPase activity in bovine lens. Pflugers Archiv European Journal of Physiology [Internet]. 1997;433(4):435–41. Available from:


Antolovic R, Kost H, Linder D, Linder M, Thönges D, Lichtstein D, et al. Demonstration of a specific transport protein for cardiac glycosides in bovine blood. Annals of the New York Academy of Sciences [Internet]. 1997;834:618–20. Available from:


Bachar C, Haver E, Kadar T, Levy A, Lichtstein D. Possible involvement of atrial natriuretic peptides in olfaction. Brain Research [Internet]. 1995;682(1–2):231–4. Available from:


Haver E, Lichtstein D, Munson PJ. Multiple types of binding sites for atrial natriuretic peptide in rat olfactory bulb membranes and synaptosomes. Brain Research [Internet]. 1995;681(1–2):75–83. Available from:


Lichtstein D, Shainberg A, Solarao J, Marban E, Ter Keurs HEDJ, Morad M. Na+, K+-ATPase and heart excitability. Advances in Experimental Medicine and Biology [Internet]. 1995;382:23–30. Available from:


Bachar H, Lichtstein D. Distribution of Atrial Natriuretic Peptides in the Sand Rat (Psammomys Obesus) in Comparison to That in the Rat. Journal of Basic and Clinical Physiology and Pharmacology [Internet]. 1993;4(1–2):47–56. Available from:


Lichtstein D, Gati I, Ovadia H. Digitalis-like compounds in the toad Bufo viridis: Interactions with plasma proteins. Journal of Cardiovascular Pharmacology [Internet]. 1993;22(SUPPL. 2):S102–5. Available from:


LICHTSTEIN D, GATI I, SAMUELOV S, BERSON D, ROZENMAN Y, LANDAU L, et al. Identification of digitalis‐like compounds in human cataractous lenses. European Journal of Biochemistry [Internet]. 1993;216(1):261–8. Available from:


Bachar H, Haver E, Ilani A, Lichtstein D. Modulation of rat olfactory bulb mitochondrial function by atrial natriuretic peptide. Pflügers Archiv European Journal of Physiology [Internet]. 1992;422(2):204–6. Available from:


Lichtstein D, Gati I, Haver E, Katz U. Digitalis-like compounds in the toad Bufo viridis: Tissue and plasma levels and significance in osmotic stress. Life Sciences [Internet]. 1992;51(2):119–28. Available from:


Lichtstein D, Gati I, Babila T, Haver E, Katz U. Effect of salt acclimation on digitalis-like compounds in the toad. BBA - General Subjects [Internet]. 1991;1073(1):65–8. Available from:


Lichtstein D, Samuelov S, Gati I, Felix AM, Gabriel TF, Deutsch J. Identification of 11,13-dihydroxy-14-octadecaenoic acid as a circulating Na+/K+-ATPase inhibitor. Journal of Endocrinology [Internet]. 1991;128(1):71–8. Available from:


Oberfrank F, Vizi ES, Baker PF, Samuelov S, Lichtstein D. Comparison of the effects of a bufodienolide and ouabain on neuronal and smooth muscle preparations. Neuroscience Research [Internet]. 1991;10(4):235–44. Available from:


Lichtstein D, Atlan H. The “cellular state”: The way to regain specificity and diversity in hormone action. Journal of Theoretical Biology [Internet]. 1990;145(3):287–94. Available from:


Babila T, Atlan H, Fromer I, Schwalb H, Uretzky G, Lichtstein D. Volume Regulation of Nerve Terminals. Journal of Neurochemistry [Internet]. 1990;55(6):2058–62. Available from:


Babila T, Gottlieb Y, Lutz RA, Lichtstein D. A bumetanide-sensitive, potassium carrier-mediated transport system in excitable tissues. Life Sciences [Internet]. 1989;44(22):1665–75. Available from:


Oberfrank F, Vizi ES, Lichtstein D. Presynaptic modulation by endogenous ouabain-like substances of noradrenaline release from blood vessels. Polish journal of pharmacology and pharmacy [Internet]. 1988;40(6):685–90. Available from:


Xu H, Lichtstein D, Kassis S, Lutz RA, Rodbard D, Chernick SS. Multiple interactions of unsaturated fatty acids with opiate and ouabain binding sites and βadrenergic sensitive adenylate cyclase system. Journal of Receptors and Signal Transduction [Internet]. 1988;8(1–4):205–23. Available from:


Bloch KD, Zamir N, Lichtstein D, Seidman CE, Seidman JG. Ouabain induces secretion of proatrial natriuretic factor by rat atrial cardiocytes. American Journal of Physiology - Endocrinology and Metabolism [Internet]. 1988;255(3):18/3. Available from:


Lichtstein D, Rodbard D. A second look at the second messenger hypothesis. Life Sciences [Internet]. 1987;40(21):2041–51. Available from:


Vizi ES, Oberfrank F, Bernath S, Lichtstein D. Noradrenaline releasing effect of an ouabain-like compound on pulmonary artery. Neuropharmacology [Internet]. 1987;26(10):1541–4. Available from:


Lutz RA, Lichtstein D, Xu H, Rodbard D. Demonstration and characterization of two classes of cardiac glycoside binding sites to rat heart membrane preparations using quantitative computer modeling. Journal of Receptors and Signal Transduction [Internet]. 1987;7(5):679–94. Available from:


Lichtstein D, Samuelov S, Deutsch J, Xu H, Lutz RA, Chernick SS, et al. The ouabain receptor in animal tissues and its endogenous ligand. Klinische Wochenschrift [Internet]. 1987;65(SUPPL. 8):40–8. Available from:


Silva P, Lear S, Segal F, Rosen S, Spokes K, Stevens A, et al. Isolated rectal gland cells: Oxygen consumption and hormonal stimulation. Mineral and Electrolyte Metabolism [Internet]. 1986;12(5–6):286–92. Available from:


Lichtstein D, Lutz RA, Xu H, Rodbard D. Characterization of two classes of cardiac glycosine binding sites in rat heart and brain membrane preparations, using quantitative computer modelling. Journal of Hypertension [Internet]. 1986;4(SUPPL. 5):S227–9. Available from:


Lichtstein D, Kachalsky S, Deutsch J. Identification of a ouabain-like compound in toad skin and plasma as a bufodienolide derivative. Life Sciences [Internet]. 1986;38(14):1261–70. Available from:


Tal DM, Katchalsky S, Lichtstein D, Karlish SJD. Endogenous “ouabain-like” activity in bovine plasma. Biochemical and Biophysical Research Communications [Internet]. 1986;135(1):1–8. Available from:


Shimoni Y, Gotsman M, Epstein M, Kachalsky S, Deutsch J, Lichtstein D. Further characterisation of the inotropic effect of a bufodienolide glycoside - an endogenous ouabain like compound. Cardiovascular Research [Internet]. 1986;20(3):229–39. Available from:


Bourrit A, Atlan H, Fromer I, Melmed RN, Lichtstein D. Basic characterization of an ouabain-resistant, bumetanide-sensitive K+ carrier-mediated transport system in J774.2 mouse macrophage-like cell line and in variants deficient in adenylate cyclase and cAMP-dependent protein kinase activities. BBA - Biomembranes [Internet]. 1985;817(1):85–94. Available from:


Lichtstein D, Samuelov S, Bourrit A. Characterization of the stimulation of neuronal Na+, K+-ATPase activity by low concentrations of ouabain. Neurochemistry International [Internet]. 1985;7(4):709–15. Available from:


Lichtstein D, Minc D, Shimoni Y, Deutsch J, Mekler J, Ben-Ishay D. Demonstration of a ouabainlike plasma compound in hypertension prone and hypertension resistant rats. Hypertension [Internet]. 1985;7(5):729–33. Available from:


Shimoni Y, Gotsman M, Deutsch J, Kachalsky S, Lichtstein D. Endogenous ouabain-like compound increases heart muscle contractility. Nature [Internet]. 1984;307(5949):369–71. Available from:


Ilani A, Yachin S, Lichtstein D. Comparison between bretylium and diphenylhydantoin interaction with mucosal sodium-channels. BBA - Biomembranes [Internet]. 1984;777(2):323–30. Available from:


Ilani A, Lichtstein D, Bacaner MB. Bretylium opens mucosal amiloride-sensitive sodium channels. BBA - Biomembranes [Internet]. 1982;693(2):503–6. Available from:


Lichtstein D, Mullikin-Kilpatrick D, Blume AJ. Modification of neuroblastoma X glioma hybrid NG108-15 adenylate cyclase by vanadium ions. Biochemical and Biophysical Research Communications [Internet]. 1982;105(3):1157–65. Available from:


Lichtstein D, Samuelov S. Characterization of ouabain receptor in neuronal tissue: Evidence for endogenous ouabain-like compound. Israel Journal of Medical Sciences [Internet]. 1982;18(1):45–50. Available from:


Lichtstein D, Mullikin-Kilpatrick D, Blume AJ. Vanadate-induced hyperpolarization of neuroblastoma-glioma hybrid NG108-15 cells. Israel Journal of Medical Sciences [Internet]. 1982;18(4):551. Available from:


Lichtstein D, Samuelov S. Membrane potential changes induced by the ouabain-like compound extracted from mammalian brain. Proceedings of the National Academy of Sciences of the United States of America [Internet]. 1982;79(5):1453–6. Available from:


Rochel S, Lichtstein D, Blume AJ, Margolis FL. Membrane potential of olfactory bulb synaptosomal fractions: Characterization with the lipophilic cation tetraphenylphosphonium. Journal of Neuroscience [Internet]. 1981;1(10):1180–92. Available from:


Lichtstein D, Samuelov S. Endogenous ‘ouabain like’ activity in rat brain. Topics in Catalysis [Internet]. 1980;96(4):1518–23. Available from: