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The Faculty of Medicine - Microbiology and Molecular Genetics: Shlomai Joseph

Researchers

 Last updated June 2021 - School of Pharmacy

List of Publications

(1) Yaffe N, Rotem D, Soni A, Porath D, Shlomai J. Direct monitoring of the stepwise condensation of kinetoplast DNA networks. Sci Rep 2021;11(1).

(2) Klebanov-Akopyan O, Mishra A, Glousker G, Tzfati Y, Shlomai J. Trypanosoma brucei UMSBP2 is a single-stranded telomeric DNA binding protein essential for chromosome end protection. Nucleic Acids Res 2018;46(15):7757-7771.

(3) Botero A, Kapeller I, Cooper C, Clode PL, Shlomai J, Thompson RCA. The kinetoplast DNA of the Australian trypanosome, Trypanosoma copemani, shares features with Trypanosoma cruzi and Trypanosoma lewisi. Int J Parasitol 2018;48(9-10):691-700.

(4) Bezalel-Buch R, Yaffe N, Shlomai J. Replication Machinery of Kinetoplast DNA. Trypanosomatid Diseases: Molecular Routes to Drug Discovery; 2013. p. 243-260.

(5) Chaki M, Airik R, Ghosh AK, Giles RH, Chen R, Slaats GG, et al. Exome capture reveals ZNF423 and CEP164 mutations, linking renal ciliopathies to DNA damage response signaling. Cell 2012;150(3):533-548.

(6) Kapeller I, Milman N, Yaffe N, Shlomai J. Interactions of a replication initiator with histone H1-like proteins remodel the condensed mitochondrial genome. J Biol Chem 2011;286(47):40566-40574.

(7) Shlomai J. Redox control of protein-DNA interactions: From molecular mechanisms to significance in signal transduction, gene expression, and DNA replication. Antioxid Redox Signal 2010;13(9):1429-1476.

(8) Liu B, Wang J, Yaffe N, Lindsay ME, Zhao Z, Zick A, et al. Trypanosomes Have Six Mitochondrial DNA Helicases with One Controlling Kinetoplast Maxicircle Replication. Mol Cell 2009;35(4):490-501.

(9) Sela D, Shlomai J. Regulation of UMSBP activities through redox-sensitive protein domains. Nucleic Acids Res 2009;37(1):279-288.

(10) Sela D, Yaffe N, Shlomai J. Enzymatic mechanism controls redox-mediated protein-DNA interactions at the replication origin of kinetoplast DNA minicircles. J Biol Chem 2008;283(46):32034-32044.

(11) Shlomai J, Sela D, Milman N, Kapeller I, Zick A, Bezalel R, et al. Unique characteristics of the kinetoplast dna replication machinery provide potential drug targets in trypanosomatids. Adv Exp Med Biol 2008;625:9-21.

(12) Milman N, Motyka SA, Englund PT, Robinson D, Shlomai J. Mitochondrial origin-binding protein UMSBP mediates DNA replication and segregation in trypanosomes. Proc Natl Acad Sci U S A 2007;104(49):19250-19255.

(13) Onn I, Kapeller I, Abu-Elneel K, Shlomai J. Binding of the universal minicircle sequence binding protein at the kinetoplast DNA replication origin. J Biol Chem 2006;281(49):37468-37476.

(14) Zick A, Onn I, Bezalel R, Margalit H, Shlomai J. Assigning functions to genes: Identification of S-phase expressed genes in Leishmania major based on post-transcriptional control elements. Nucleic Acids Res 2005;33(13):4235-4242.

(15) Shlomai J. The structure and replication of kinetoplast DNA. Curr Mol Med 2004;4(6):623-647.

(16) Onn I, Milman-Shtepel N, Shlomai J. Redox potential regulates binding of universal minicircle sequence binding protein at the kinetoplast DNA replication origin. Eukaryotic Cell 2004;3(2):277-287.

(17) Shlomai J. Specific recognition of the replication origins of the kinetoplast DNA. Acta Microbiol Immunol Hung 2002;49(4):455-467.

(18) Abu-Elneel K, Robinson DR, Drew ME, Englund PT, Shlomai J. Intramitochondrial localization of universal minicircle sequence-binding protein, a trypanosomatid protein that binds kinetoplast minicircle replication origins. J Cell Biol 2001;153(4):725-733.

(19) Abu-Elneel K, Kapeller I, Shlomai J. Universal minicircle sequence-binding protein, a sequence-specific DNA- binding protein that recognizes the two replication origins of the kinetoplast DNA minicircle. J Biol Chem 1999;274(19):13419-13426.

(20) Tzfati Y, Shlomai J. Genomic organization and expression of the gene encoding the universal minicircle sequence binding protein. Mol Biochem Parasitol 1998;94(1):137-141.

(21) Flashner Y, Shlomai J, Shafferman A. Three novel plasmid R6K proteins act in concert to distort DNA within the α and β origins of DNA replication. Mol Microbiol 1996;19(5):985-996.

(22) Avrahami D, Tzfati Y, Shlomai J. A single-stranded DNA binding protein binds the origin of replication of the duplex kinetoplast DNA. Proc Natl Acad Sci U S A 1995;92(23):10511-10515.

(23) Abeliovich H, Shlomai J. Reversible oxidative aggregation obstructs specific proteolytic cleavage of glutathione S-transferase fusion proteins. Anal Biochem 1995;228(2):351-354.

(24) Tzfati Y, Abeliovich H, Avrahami D, Shlomai J. Universal minicircle sequence binding protein, a CCHC-type zinc finger protein that binds the universal minicircle sequence of trypanosomatids. Purification and characterization. J Biol Chem 1995;270(36):21339-21345.

(25) Shlomai J. The assembly of kinetoplast DNA. Parasitol Today 1994;10(9):341-346.

(26) Abeliovich H, Tzfati Y, Shlomai J. A trypanosomal CCHC-type zinc finger protein which binds the conserved universal sequence of kinetoplast DNA minicircles: Isolation and analysis of the complete cDNA from Crithidia fasciculata. Mol Cell Biol 1993;13(12):7766-7773.

(27) Masuda ES, Tokumitsu H, Tsuboi A, Shlomai J, Hung P, Arai K-, et al. The granulocyte-macrophage colony-stimulating factor promoter cis-acting element CLE0 mediates induction signals in T cells and is recognized by factors related to AP1 and NFAT. Mol Cell Biol 1993;13(12):7399-7407.

(28) Tzfati Y, Abeliovich H, Kapeller I, Shlomai J. A single-stranded DNA-binding protein from Crithidia fasciculata recognizes the nucleotide sequence at the origin of replication of kinetoplast DNA minicircles. Proc Natl Acad Sci U S A 1992;89(15):6891-6895.

(29) Schlein Y, Jacobson RL, Shlomai J. Chitinase secreted by Leishmania functions in the sandfly vector. Proc R Soc B Biol Sci 1991;245(1313):121-126.

(30) Miyatake S, Shlomai J, Arai K-, Arai N. Characterization of the mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) gene promoter: Nuclear factors that interact with an element shared by three lymphokine genes - Those for GM-CSF, interleukin-4 (IL-4), and IL-5. Mol Cell Biol 1991;11(12):5894-5901.

(31) Arai N, Tsuboi A, Iwai Y, Miyatake S, Yokota K, De Waal Malefyt R, et al. Regulation of IL-3, IL-4 and GM-CSF genes and signal transduction by their receptors. Lymphokine Res 1990;9(4):551-553.

(32) Linial M, Shlomai J. Bent DNA structures associated with several origins of replication are recognized by a unique enzyme from trypanosomatids. Nucleic Acids Res 1988;16(14):6477-6492.

(33) Miyatake S, Seiki M, Dewaal Malefijt R, Heike T, Fujisawa J-, Takebe Y, et al. Activation of T cell-derived lymphokine genes in T cells and fibroblasts: Effects of human T cell leukemia virus type I P40x protein and bovine papilloma virus encoded E2 protein. Nucleic Acids Res 1988;16(14):6547-6566.

(34) Linial M, Shlomai J. A unique endonuclease from Crithidia fasciculata which recognizes a bend in the DNA helix. Specificity of the cleavage reaction. J Biol Chem 1988;263(1):290-297.

(35) Linial M, Shlomai J. The sequence-directed bent structure in kinetoplast DNA is recognized by an enzyme from Crithidia fasciculata. J Biol Chem 1987;262(31):15194-15201.

(36) Linial M, Shlomai J. Sequence-directed bent DNA helix is the specific binding site for Crithidia fasciculata nicking enzyme. Proc Natl Acad Sci U S A 1987;84(23):8205-8209.

(37) Shlomai J, Linial M. A nicking enzyme from trypanosomatids which specifically affects the topological linking of duplex DNA circles. Purification and characterization. J Biol Chem 1986;261(34):16219-16225.

(38) Millgram MA, Greenblatt CL, Shlomai J. Interaction of aminoglycosides and ionophores in the killing of Crithidia fasciculata. Z Parasitenkd 1985;71(6):699-704.

(39) Shlomai J, Zadok A. Kinetoplast DNA minicircles of trypanosomatids encode for a protein product. Nucleic Acids Res 1984;12(21):8017-8028.

(40) Shlomai J, Zadok A, Frank D. A unique ATP-dependent DNA topoisomerase from trypanosomatids. Adv Exp Med Biol 1984;179:409-422.

(41) Shlomai J, Zadok A. Reversible decatenation of kinetoplast DNA by a DNA topoisomerase from trypanosomatids. Nucleic Acids Res 1983;11(12):4019-4034.

(42) Schlein Y, Warburg A, Schnur LF, Shlomai J. Vector compatibility of Phlebotomus papatasi dependent on differentially induced digestion. Acta Trop 1983;40(1):65-70.

(43) Low RL, Shlomai J, Kornberg A. Protein n, a primosomal DNA replication protein of Escherichia coli: Purification and characterization. J Biol Chem 1982;257(11):6242-6250.

(44) Millgram M, Greenblatt CL, Shlomai J, Kaback HR. The interaction of ionophores and aminoglycosides in the killing of Crithidia. J Protozool 1982;29(3):536.

(45) Arai K, Low R, Kobori J, Shlomai J, Kornberg A. Mechanism of dnaB protein action. V. Association of dnaB protein, protein n', and other prepriming proteins in the primosome of DNA replication. J Biol Chem 1981;256(10):5273-5280.

(46) Shlomai J, Polder L, Arai K, Kornberg A. Replication of ∅X174 DNA with purified enzymes. I. Conversion of viral DNA to a supercoiled, biologically active duplex. J Biol Chem 1981;256(10):5233-5238.

(47) Arai K-, Arai N, Shlomai J, Kobori J, Polder L, Low R, et al. Enzyme Studies of ϕX174 DNA Replication. Prog Nucleic Acid Res Mol Biol 1981;26(C):9-32.

(48) Shlomai J, Kornberg A. A prepriming DNA replication enzyme of Escherichia coli. II. Actions of protein n': a sequence-specific, DNA-dependent ATPase. J Biol Chem 1980;255(14):6794-6798.

(49) Shlomai J, Kornberg A. A prepriming DNA replication enzyme of Escherichia coli. I. Purification of protein n': a sequence-specific, DNA-dependent ATPase. J Biol Chem 1980;255(14):6789-6793.

(50) Shlomai J, Kornberg A. An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in φX174 DNA. Proc Natl Acad Sci U S A 1980;77(2 II):799-803.

(51) Arai K, Arai N, Shlomai J, Kornberg A. Replication of duplex DNA of phage φX174 reconstituted with purified enzymes. Proc Natl Acad Sci U S A 1980;77(6 I):3322-3326.

(52) Meyer RR, Shlomai J, Kobori J, Bates DL, Rowen L, McMacken R, et al. Enzymatic conversion of single-stranded phiX174 and G4 circles to duplex forms: Discontinuous replication. COLD SPRING HARBOR SYMP QUANT BIOL 1979;43(1):289-293.

(53) Shlomai J, Kornberg A. Deoxyuridine triphosphatase of Escherichia coli. Purification, properties, and use as a reagent to reduce uracil incorporation into DNA. J Biol Chem 1978;253(9):3305-3312.

(54) Shlomai J, Becker Y. Replication of herpes simplex virus DNA after removal of hydroxyurea block from infected cells. J Gen Virol 1977;37(2):429-433.

(55) Friedmann A, Shlomai J, Becker Y. Electron microscopy of herpes simplex virus DNA molecules isolated from infected cells by centrifugation in CsCl density gradients. J Gen Virol 1977;34(3):507-522.

(56) Shlomai J, Asher Y, Becker Y. In vitro synthesis of herpes simplex virus DNA in nuclei isolated from infected BSC1 cells. J Gen Virol 1977;34(2):223-234.

(57) Becker Y, Asher Y, Cohen Y, Weinberg-Zahlering E, Shlomai J. Phosphonoacetic acid-resistant mutants of herpes simplex virus: effect of phosphonoacetic acid on virus replication and in vitro deoxyribonucleic acid synthesis in isolated nuclei. Antimicrob Agents Chemother 1977;11(5):919-922.

(58) Shlomai J, Friedmann A, Becker Y. Replicative intermediates of herpes simplex virus DNA. Virology 1976;69(2):647-659.

(59) Shlomai J, Strauss B, Asher Y, Friedmann A, Becker Y. Analysis of herpes simplex virus DNA synthesized in infected nuclei by chromatography on benzoylated naphthoylated DEAE cellulose columns. J Gen Virol 1976;32(2):189-204.

(60) Becker Y, Ben-Zeev A, Kamincik Y, Asher Y, Shlomai J. Nucleic acid biosynthesis in nuclei of cells infected with herpesviruses (HSV and EBV). IARC Sci Publ 1975;No.11 I:245-257.

(61) Shlomai J, Becker Y. Analysis of herpes simplex virus DNA synthesized in vitro in nuclei isolated from infected BSC-1 cells. Virology 1975;68(1):275-279.

(62) Shlomai J, Asher Y, Gordon YJ, Olshevsky U, Becker Y. Effect of zinc ions on the synthesis of herpes simplex virus DNA in infected BSC-1 cells. Virology 1975;66(1):330-335.

(63) Becker Y, Shlomai J, Weinberg E, Ben-Zeev A, Olshevsky U. Integration of Epstein-Barr virus DNA into cellular DNA of Burkitt lymphoblasts (Raji cell line). Isr J Med Sci 1974;10(11):1454-1457.

(64) Becker Y, Shlomai J, Asher Y, Weinberg E, Cohen Y, Olshevsky U, et al. Interaction of herpes simplex virus type 1 with rous sarcoma virus-transformed rat cells [XC and r(B77) cell lines]. Intervirology 1974;4(6):325-332.