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The Faculty of Medicine - Biochemistry and Molecular Biology: Kaempfer Raymond

Researchers

 Last updated June 2021 - School of Pharmacy

List of Publications

(1) Edgar R, Cohen A, Hillman D, Kaempfer R, Shirvan A. Prolonged Benefit of Reltecimod Despite Short Plasma Half-Life. Int J Pept Res Ther 2020;26(4):2399-2410.

(2) Edgar R, Tarrio ML, Maislin G, Chiguang F, Kaempfer R, Cross A, et al. Treatment with One Dose of Reltecimod is Superior to Two Doses in Mouse Models of Lethal Infection. Int J Pept Res Ther 2020;26(3):1669-1683.

(3) Klepsch O, Namer LS, Köhler N, Kaempfer R, Dittrich A, Schaper F. Intragenic regulation of SOCS3 isoforms. Cell Commun Signal 2019;17(1).

(4) Popugailo A, Rotfogel Z, Supper E, Hillman D, Kaempfer R. Staphylococcal and streptococcal superantigens trigger B7/CD28 costimulatory receptor engagement to hyperinduce inflammatory cytokines. Front Immunol 2019;10(APR).

(5) Kaempfer R, Ilan L, Cohen-Chalamish S, Turgeman O, Sarah Namer L, Osman F. Control of mRNA splicing by intragenic RNA activators of stress signaling: Potential implications for human disease. Front Genet 2019;10(MAY).

(6) Kaempfer R, Namer LS, Osman F, Ilan L. Control of mRNA splicing by noncoding intragenic RNA elements that evoke a cellular stress response. Int J Biochem Cell Biol 2018;105:20-23.

(7) Kaempfer R. Bacterial superantigen toxins, CD28, and drug development. Toxins 2018;10(11).

(8) Kaempfer R. Ribosome cycle emerges from DNA replication. Nat Rev Mol Cell Biol 2017;18(8):470.

(9) Namer LS, Osman F, Banai Y, Masquida B, Jung R, Kaempfer R. An Ancient Pseudoknot in TNF-α Pre-mRNA Activates PKR, Inducing eIF2α Phosphorylation that Potently Enhances Splicing. Cell Rep 2017;20(1):188-200.

(10) Ilan L, Osman F, Namer LS, Eliahu E, Cohen-Chalamish S, Ben-Asouli Y, et al. PKR activation and eIF2α phosphorylation mediate human globin mRNA splicing at spliceosome assembly. Cell Res 2017;27(5):688-704.

(11) Levy R, Rotfogel Z, Hillman D, Popugailo A, Arad G, Supper E, et al. Superantigens hyperinduce inflammatory cytokines by enhancing the B7-2/CD28 costimulatory receptor interaction. Proc Natl Acad Sci U S A 2016;113(42):E6437-E6446.

(12) Arad G, Levy R, Nasie I, Hillman D, Rotfogel Z, Barash U, et al. Correction to Binding of Superantigen Toxins into the CD28 Homodimer Interface Is Essential for Induction of Cytokine Genes That Mediate Lethal Shock [PLoS Biol, (2015), 13, 8]. PloS Biol 2015;13(8).

(13) Ramachandran G, Kaempfer R, Chung C-, Shirvan A, Chahin AB, Palardy JE, et al. CD28 homodimer interface mimetic peptide acts as a preventive and therapeutic agent in models of severe bacterial sepsis and gram-negative bacterial peritonitis. J Infect Dis 2015;211(6):995-1003.

(14) Mirzoeva S, Paunesku T, Wanzer MB, Shirvan A, Kaempfer R, Woloschak GE, et al. Single administration of p2TA (AB103), a CD28 antagonist peptide, prevents inflammatory and thrombotic reactions and protects against gastrointestinal injury in total-body irradiated mice. PLoS ONE 2014;9(7).

(15) Bulger EM, Maier RV, Sperry J, Joshi M, Henry S, Moore FA, et al. A novel drug for treatment of necrotizing soft-tissue infections: A randomized clinical trial. JAMA Surg 2014;149(6):528-536.

(16) Kaempfer R, Arad G, Levy R, Hillman D, Nasie I, Rotfogel Z. CD28: Direct and critical receptor for superantigen toxins. Toxins 2013;5(9):1531-1542.

(17) Ramachandran G, Tulapurkar ME, Harris KM, Arad G, Shirvan A, Shemesh R, et al. A peptide antagonist of CD28 signaling attenuates toxic shock and necrotizing soft-tissue infection induced by streptococcus pyogenes. J Infect Dis 2013;207(12):1869-1877.

(18) Kaempfer R. Kick-starting the origin of life. Comment on "Formamide and the origin of life" by R. Saladino, C. Crestini, S. Pino, G. Costanzo and E. Di Mauro. Phys Life Rev 2012;9(1):111-113.

(19) Arad G, Levy R, Nasie I, Hillman D, Rotfogel Z, Barash U, et al. Binding of superantigen toxins into the CD28 homodimer interface is essential for induction of cytokine genes that mediate lethal shock. PloS Biol 2011;9(9).

(20) Cohen-Chalamish S, Hasson A, Weinberg D, Namer LS, Banai Y, Osman F, et al. Dynamic refolding of IFN-γ mRNA enables it to function as PKR activator and translation template. Nat Chem Biol 2009;5(12):896-903.

(21) Kaempfer R. Interferon-γ mRNA attenuates its own translation by activating PKR: A molecular basis for the therapeutic effect of interferon-β in multiple sclerosis. Cell Res 2006;16(2):148-153.

(22) Kaempfer R. Peptide antagonists of superantigen toxins. Mol Diversity 2004;8(2):113-120.

(23) Arad G, Hillman D, Levy R, Kaempfer R. Broad-spectrum immunity against superantigens is elicited in mice protected from lethal shock by a superantigen antagonist peptide. Immunol Lett 2004;91(2-3):141-145.

(24) Kaempfer R. RNA sensors: Novel regulators of gene expression. EMBO Rep 2003;4(11):1043-1047.

(25) Kaempfer R, Arad G, Levy R, Hillman D. Defense against biologic warfare with superantigen toxins. Isr Med Assoc J 2002;4(7):520-523.

(26) Arad G, Levy R, Kaempfer R. Superantigen concomitantly induces Th1 cytokine genes and the ability to shut off their expression on re-exposure to superantigen. Immunol Lett 2002;82(1-2):75-78.

(27) Ben-Asouli Y, Banai Y, Pel-Or Y, Shir A, Kaempfer R. Human interferon-γ mRNA autoregulates its translation through a pseudoknot that activates the interferon-inducible protein kinase PKR. Cell 2002;108(2):221-232.

(28) Arad G, Hillman D, Levy R, Kaempfer R. Superantigen antagonist blocks Th1 cytokine gene induction and lethal shock. J Leukocyte Biol 2001;69(6):921-927.

(29) Schneider R, Agol VI, Andino R, Bayard F, Cavener DR, Chappell SA, et al. New ways of initiating translation in eukaryotes? [2](multiple letters). Mol Cell Biol 2001;21(23):8238-8246.

(30) Arad G, Levy R, Hillman D, Kaempfer R. Superantigen antagonist protects against lethal shock and defines a new domain for T-cell activation. Nat Med 2000;6(4):414-421.

(31) Ben-Asouli Y, Banai Y, Hauser H, Kaempfer R. Recognition of 5'-terminal TAR structure in human immunodeficiency virus-1 mRNA by eukaryotic translation initiation factor 2. Nucleic Acids Res 2000;28(4):1011-1018.

(32) Richmond A, Kaempfer R. Cytokines revisited at Hilton Head. Cytokine Growth Factor Rev 2000;11(3):255-266.

(33) Osman F, Jarrous N, Ben-Asouli Y, Kaempfer R. A cis-acting element in the 3'-untranslated region of human TNF-α mRNA renders splicing dependent on the activation of protein kinase PKR. Genes Dev 1999;13(24):3280-3293.

(34) Ketzinel M, Kaempfer R. Cell-mediated suppression of human interleukin-2 gene expression at splicing of mRNA. Immunol Lett 1999;68(1):161-166.

(35) Kaempfer R. Cytokine and interferon research in Israel. Cytokine Growth Factor Rev 1998;9(2):99-108.

(36) Gerez L, Shkolnik T, Hirschmann O, Lorber M, Arad G, Kaempfer R. Hyperinducible expression of the interferon-gamma (IFN-γ) gene and its suppression in systemic lupus erythematosus (SLE). Clin Exp Immunol 1997;109(2):296-303.

(37) Aframian D, Katzenellenbogen M, Arad G, Osman F, Sayar D, Ketzinel M, et al. Down-regulation of human tumor necrosis factor-β gene expression by cells with suppressive activity. Immunol Lett 1996;54(2-3):171-176.

(38) Arad G, Katzenellenbogen M, Levy R, Slavin S, Kaempfer R. Linomide, an immunomodulator that inhibits T(h)1 cytokine gene expression. Int Immunol 1996;8(10):1603-1607.

(39) Arad G, Nussinovich R, Na'Amad M, Kaempfer R. Dual control of human interleukin-2 and interferon-γ gene expression by histamine: Activation and suppression. Cell Immunol 1996;170(1):149-155.

(40) Jarrous N, Osman F, Kaempfer R. 2-Aminopurine selectively inhibits splicing of tumor necrosis factor alpha mRNA. Mol Cell Biol 1996;16(6):2814-2822.

(41) Kaempfer R, Gerez L, Farbstein H, Madar L, Hirschman O, Nussinovich R, et al. Prediction of response to treatment in superficial bladder carcinoma through pattern of interleukin-2 gene expression. J Clin Oncol 1996;14(6):1778-1786.

(42) Arad G, Nussinovich R, Kaempfer R. Interleukin-2 induces an early step in the activation of interferon-γ gene expression. Immunol Lett 1995;44(2-3):213-216.

(43) Gerez L, Arad G, Efrat S, Ketzinel M, Kaempfer R. Post-transcriptional regulation of human interleukin-2 gene expression at processing of precursor transcripts. J Biol Chem 1995;270(33):19569-19575.

(44) Arad G, Ketzinel M, Tal C, Nussinovich R, Deutsch E, Schlesinger M, et al. Transient expression of human interleukin-2 and interferon-γ genes is regulated by interaction between distinct cell subsets. Cell Immunol 1995;160(2):240-247.

(45) Jarrous N, Kaempfer R. Induction of human interleukin-1 gene expression by retinoic acid and its regulation at processing of precursor transcripts. J Biol Chem 1994;269(37):23141-23149.

(46) Kaempfer R. Regulation of the Human Interleukin-2/Interleukin-2 Receptor System: A Role for Immunosuppression. Proc Soc Exp Biol Med 1994;206(3):176-180.

(47) Gonsky R, Itamar D, Harary R, Kaempfer R. Binding of ATP and messenger RNA by the β-subunit of eukaryotic initiation factor 2. Biochimie 1992;74(5):427-434.

(48) KETZINEL M, EFRAT S, SAYAR D, GEREZ L, TAL C, DEUTSCH E, et al. Regulation of Human Interleukin‐2 and Interferon‐Gamma Gene Expression by Suppressor T Lymphocytes. Scand J Immunol 1991;33(5):593-605.

(49) Gerez L, Madar L, Arad G, Sharav T, Reshef A, Ketzinel M, et al. Aberrant regulation of interleukin-2 but not of interferon-γ gene expression in Down syndrome (trisomy 21). Clin Immunol Immunopathol 1991;58(2):251-266.

(50) Gerez L, Madar L, Shkolnik T, Kristal B, Arad G, Reshef A, et al. Regulation of interleukin-2 and interferon-γ gene expression in renal failure. Kidney Int 1991;40(2):266-272.

(51) Harary R, Kaempfer R. Distinct epitopes in eukaryotic initiation factor 2 for binding of mRNA and for ternary complex formation with methionyl-tRNAf and GTP. Biochim Biophys Acta Gene Struct Expr 1990;1050(1-3):129-133.

(52) Gonsky R, Lebendiker MA, Harary R, Banai Y, Kaempfer R. Binding of ATP to eukaryotic initiation factor 2. Differential modulation of mRNA-binding activity and GTP-dependent binding of methionyl-tRNA(f/Met). J Biol Chem 1990;265(16):9083-9089.

(53) Manor D, Nussinovich R, Yeheskel A, Kaempfer R. A constitutive antibody in normal human serum directed against rabbit bone marrow cells: Lack in parturients, neonates, and hematologic disorders. J Lab Clin Med 1990;116(6):771-778.

(54) Harary R, Gonsky R, Itamar D, Kaempfer R. Relief of cytotoxicity and enhancement of interferon inducer activity of double-stranded RNA by eukaryotic initiation factor 2. Virology 1990;174(2):494-503.

(55) Sayar D, Ketzinel M, Gerez L, Silberberg C, Reshef A, Kaempfer R. Expression of the human IL-2 receptor on lymphocytes involves rapid turnover of its p55 α-subunit (Tac). J Immunol 1990;145(9):2946-2949.

(56) Ketzinel M, Gila A, Tal C, Schlesinger M, Nussinovich R, Reshef A, et al. The potential to express or suppress human interleukin-2 and interferon-γ genes is not restricted to distinct cell subsets. Mol Immunol 1990;27(12):1325-1330.

(57) Leitersdorf E, Banai Y, Friedman G, Kaempfer R. Superinduction of the human gene encoding low density lipoprotein receptor. Biochem Biophys Res Commun 1989;165(2):574-580.

(58) Lebendiker MA, Tal C, Sayar D, Pilo S, Eilon A, Banai Y, et al. Superinduction of the human gene encoding immune interferon. EMBO J 1987;6(3):585-589.

(59) Rosenthal A, Marsh S, Manor D, Kaempfer R. DNA synthesis by erythroid precursors in a completely defined medium: A rapid, sensitive, and convenient bioassay for erythropoietin. Exp Hematol 1985;13(3):174-184.

(60) Efrat S, Kaempfer R. A qualitative difference in the interleukin 2 (IL-2) requirement of helper and cytotoxic T lymphocytes. Cell Immunol 1984;88(1):207-212.

(61) Efrat S, Zelig S, Yagen B, Kaempfer R. Superinduction of human interleukin-2 messenger RNA by inhibitors of translation. Biochem Biophys Res Commun 1984;123(2):842-848.

(62) Efrat S, Kaempfer R. Control of biologically active interleukin 2 messenger RNA formation in induced human lymphocytes. Proc Natl Acad Sci U S A 1984;81(9 I):2601-2605.

(63) Knoller S, Kaempfer R. Isolation of a Heme-Controlled Inhibitor of Translation That Blocks the Interaction between Messenger RNA and Eukaryotic Initiation Factor 2. Biochemistry 1984;23(11):2462-2469.

(64) ITAMAR D, GONSKY R, LEBENDIKER M, KAEMPFER R. The nature of the interaction of eukaryotic initiation factor 2 with double‐stranded RNA. Eur J Biochem 1984;145(2):373-379.

(65) KAEMPFER R, KONIJN AM. Translational Competition by mRNA Species Encoding Albumin, Ferritin, Haemopexin and Globin. Eur J Biochem 1983;131(3):545-550.

(66) Efrat S, Pilo S, Kaempfer R. Kinetics of induction and molecular size of mRNAs encoding human interleukin-2 and γ-interferon. Nature 1982;297(5863):236-239.

(67) Rosen H, Di Segni G, Kaempfer R. Translational control by messenger RNA competition for eukaryotic initiation factor 2. J Biol Chem 1982;257(2):946-952.

(68) Perez-Bercoff R, Kaempfer R. Genomic RNA of mengovirus. V. Recognition of common features by ribosomes and eucaryotic initiation factor 2. J Virol 1982;41(1):30-41.

(69) Kaempfer R, Efrat S, Pilo S. Expression and molecular size of messenger RNA species encoding interleukin-2 and gamma interferon from normal human lymphocytes. Fed Proc 1982;41(4):4018.

(70) Kaempfer R, Van Emmelo J, Fiers W. Specific binding of eukaryotic initiation factor 2 to satellite tobacco necrosis virus RNA at a 5'-terminal sequence comprising the ribosome binding site. Proc Natl Acad Sci U S A 1981;78(3 I):1542-1546.

(71) Rosen H, Knoller S, Kaempfer R. Messenger Ribonucleic Acid Specificity in the Inhibition of Eukaryotic Translation by Double-Stranded Ribonucleic Acid. Biochemistry 1981;20(11):3011-3020.

(72) Rosen H, Kaempfer R. Mutually exclusive binding of messenger RNA and initiator methionyl transfer RNA to eukaryotic initiation factor 2. Biochem Biophys Res Commun 1979;91(2):449-455.

(73) Kaempfer R. RNA-Affinity Chromatography: Its Use in Purification of Eukaryotic Initiation Factor 2. Methods Enzymol 1979;60(C):247-255.

(74) Kaempfer R. Binding of Messenger RNA in Initiation of Eukaryotic Translation. Methods Enzymol 1979;60(C):380-392.

(75) Kaempfer R, Jay G. Binding of Messenger RNA in Initiation of Prokaryotic Translation. Methods Enzymol 1979;60(C):332-343.

(76) Segni GD, Rosen H, Kaempfer R. Competition between α- and β-Globin Messenger Ribonucleic Acids for Eucaryotic Initiation Factor 2. Biochemistry 1979;18(13):2847-2854.

(77) KAEMPFER R, HOLLENDER R, SOREQ H, NUDEL U. Recognition of Messenger RNA in Eukaryotic Protein Synthesis: Equilibrium Studies of the Interaction between Messenger RNA and the Initiation Factor that Binds Methionyl‐tRNAf. Eur J Biochem 1979;94(2):591-600.

(78) Kaempfer R, Israeli R, Rosaeli H, Knoller S, Zilberstein A, Schmidt A, et al. Reversal of the interferon-induced block of protein synthesis by purified preparations of eucaryotic initiation factor 2. Virology 1979;99(1):170-173.

(79) Di Segni G, Kerem H, Cividalli G, Rachmilewitz EA, Kaempfer R. Absence of functional β-globin messenger RNA in Kurdish Jews with β 0-thalassemia. Isr J Med Sci 1978;14(11):1116-1123.

(80) Kaempfer R, Rosen H, Israeli R. Translational control: Recognition of the methylated 5' end and an internal sequence in eukaryotic mRNA by the initiation factor that binds methionyl-tRNA(f)(met). Proc Natl Acad Sci U S A 1978;75(2):650-654.

(81) Kaempfer R, Hollender R, Abrams WR, Israeli R. Specific binding of messenger RNA and methionyl-tRNA(f)(Met) by the same initiation factor for eukaryotic protein synthesis. Proc Natl Acad Sci U S A 1978;75(1):209-213.

(82) Izak G, Karsai A, Cohen S, Kaempfer R. Observations on erythroid cell differentiation and maturation in synchronized rabbit erythropoetic tissue. NEW ISTANB CONTR CLIN SCI 1977;12(1):34-42.

(83) Jay G, Kaempfer R. Translational repression of a viral messenger RNA by a host protein. J Biol Chem 1975;250(15):5749-5755.

(84) Jay G, Kaempfer R. Initiation of protein synthesis. Binding of messenger RNA. J Biol Chem 1975;250(15):5742-5748.

(85) Leick VR, Santerre RF, Kaempfer R. Masking of peptidyl transferase activity in polyribosomes. Arch Biochem Biophys 1975;169(2):622-626.

(86) Kaempfer R. Identification and RNA-binding properties of an initiation factor capable of relieving translational inhibition induced by heme deprivation or double-stranded RNA. Biochem Biophys Res Commun 1974;61(2):591-597.

(87) Jay G, Abrams WR, Kaempfer R. Resistance of bacterial protein synthesis to double-stranded RNA. Biochem Biophys Res Commun 1974;60(4):1357-1364.

(88) Jay G, Kaempfer R. Host interference with viral gene expression: Mode of action of bacterial factor i. J Mol Biol 1974;82(2):193-212.

(89) Jay G, Kaempfer R. Sequence of events in initiation of translation: a role for initiator transfer RNA in the recognition of messenger RNA. Proc Natl Acad Sci U S A 1974;71(8):3199-3203.

(90) Raffel C, Stein S, Kaempfer R. Role for heme in mammalian protein synthesis: activation of an initiation factor. Proc Natl Acad Sci U S A 1974;71(10):4020-4024.

(91) Kaempfer R, Kaufman J. Inhibition of cellular protein synthesis by double-stranded RNA: inactivation of an initiation factor. Proc Natl Acad Sci U S A 1973;70(4):1222-1226.

(92) Kaempfer R. Initiation factor IF-3: a specific inhibitor of ribosomal subunit association. J Mol Biol 1972;71(3):583-598.

(93) Kaempfer R, Kaufman J. Translational control of hemoglobin synthesis by an initiation factor required for recycling of ribosomes and for their binding to messenger RNA. Proc Natl Acad Sci U S A 1972;69(11):3317-3321.

(94) Kaempfer R. Ribosomal Subunit Exchange: Analysis in Vivo. Methods Enzymol 1971;20(C):456-467.

(95) Kaempfer R. Ribosomal Subunit Exchange: Analysis in Vitro. Methods Enzymol 1971;20(C):467-472.

(96) Kaempfer R. Control of single ribosome formation by an initiation factor for protein synthesis. Proc Natl Acad Sci U S A 1971;68(10):2458-2462.

(97) Kaempfer R, Meselson M. Sedimentation Velocity Analysis in Accelerating Gradients. Methods Enzymol 1971;20(C):521-528.

(98) Kaempfer R. Dissociation of ribosomes on polypeptide chain termination and origin of single ribosomes. Nature 1970;228(5271):534-537.

(99) Kaempfer R. Ribosomal subunit exchange in the cytoplasm of a eukaryote. Nature 1969;222(5197):950-953.

(100) Kaempfer R, Meselson M. Studies of ribosomal subunit exchange. Cold Spring Harb Symp Quant Biol 1969;34:209-220.

(101) Kaempfer R, Meselson M. Permanent association of 5 s RNA molecules with 50 s ribosomal subunits in growing bacteria. J Mol Biol 1968;34(3):703-707.

(102) Kaempfer ROR, Meselson M, Raskas HJ. Cyclic dissociation into stable subunits and re-formation of ribosomes during bacterial growth. J Mol Biol 1968;31(2):277-289.

(103) Kaempfer R. Ribosomal subunit exchange during protein synthesis. Proc Natl Acad Sci U S A 1968;61(1):106-113.

(104) Kaempfer ROR, Sarkar S. Effect of infection with T-even phage on the constitutive synthesis of β-galactosidase in Escherichia coli. J Mol Biol 1967;27(3):469-474.

(105) Kaempfer ROR, Magasanik B. Effect of infection with T-even phage on the inducible synthesis of β-galactosidase in Escherichia coli. J Mol Biol 1967;27(3):453-468.

(106) Kaempfer ROR, Magasanik B. Mechanism of β-galactosidase induction in Escherichia coli. J Mol Biol 1967;27(3):475-494.

(107) Bilezikian JP, Kaempfer ROR, Magasanik B. Mechanism of tryptophanase induction in Escherichia coli. J Mol Biol 1967;27(3):495-506.